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KMID : 1377020230200030435
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Tissue Engineering and Regenerative Medicine
2023 Volume.20 No. 3 p.435 ~ p.446
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Pathological Characteristics of Monosodium Iodoacetate-Induced Osteoarthritis in Rats
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Kwon Min-Ji
Nam Dong-Yeon
Kim June-Sun
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Abstract
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BACKGROUND : This study aimed to identify pain-related behavior and pathological characteristics of the knee joint in rats with monosodium iodoacetate (MIA)-induced osteoarthritis (OA).
METHODS : Knee joint inflammation was induced by intra-articular injection of MIA (4 mg/50 μL, n?=?14) in 6-week-old male rats. Knee joint diameter, weight-bearing percentage on the hind limb during walking, the knee bending score, and paw withdrawal to mechanical stimuli were measured to evaluate edema and pain-related behavior for 28 d after MIA injection. Histological changes in the knee joints were evaluated using safranin O fast green staining on days 1, 3, 5, 7, 14, and 28 after OA induction (n?=?3, respectively). Changes in bone structure and bone mineral density (BMD) were examined 14 and 28 d after OA (n?=?3, respectively) using micro-computed tomography (CT).
RESULTS : The knee joint diameter and knee bending scores of the ipsilateral joint significantly increased 1 d after MIA injection, and the increased knee joint diameter and knee bending score persisted for 28 d. Weight-bearing during walking and paw withdrawal threshold (PWT) decreased from 1 and 5 d, respectively, and were maintained up to 28 d after MIA. Cartilage destruction started on day 1, and Mankin scores for bone destruction significantly increased for 14 d, as shown by micro-CT imaging.
CONCLUSION : The present study demonstrated that histopathological structural changes in the knee joint due to inflammation started soon after MIA injection, which induced OA pain from inflammation-related acute pain to spontaneous and evoked associated chronic pain.
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KEYWORD
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Osteoarthritis, Monosodium iodoacetate model, Articular cartilage, Arthritic pain, Histopathology
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